Spectrum CE System Operating Manual

Instructions for Use of Product(s)
CE1008, CE1024

Literature # TMD052

The Spectrum CE System is an automated capillary electrophoresis instrument intended for separation and detection of fluorescently labeled DNA fragments, including short tandem repeat (STR) markers. This instrument is available in two formats: An 8-capillary system (Cat.# CE1008) and a 24-capillary system (Cat.# CE1024). The Spectrum CE System is designed for use by laboratory professionals, including forensic laboratories. The CE instrument is compatible with existing four-, five- and six-dye-labeled STR kits available from Promega and other commercial vendors. It is also compatible with the Promega eight-dye STR kits. The system supports automated analysis of up to four 96-well sample plates.

The system includes the capillary electrophoresis instrument, desktop computer, monitor and consumables. The instrument is controlled through a graphical user interface running on the system computer. The Spectrum Control Software (SCS) provides a simple user interface with a clear display of useful features including run setup, consumables usage information, and system maintenance reminders. The integrated RFID readers are used to capture RFID information on consumables for easy exchange and tracking of instrument consumables.

Summary of Changes
The following changes were made to the 8/26 revision of this document:
1. Added the Spectrum CE System, 24-Capillary, to this document in Sections 1.1, 1.6, 1.7, 3.1, 4.2.1, 6.1.1, 10.3 and 10.4.
2. Updated Section 2.4 and included a new plate mapping illustration.
3. Retitled Section 3.1.
4. Inserted new Section 3.2, including instructions previously part of Section 3.1, and added Section 3.3.
5. Added text and Calibration Status table previously in Section 3.2 to Section 3.4 and inserted a new figure above note on page 43.
6. Corrected Section 4.1.
7. Updated text in Sections 6.2.2, 9.2 and 10.5.

Revised 8/26.

Complete Protocol
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