Human Mixture Sample Analysis on the Spectrum Compact CE System
Katalognummer GPN059
Abstract
Leta S. Steffen , Nicole King
Global Support Services, Promega Corporation
Human sample identification is used for confirming twin zygosity, biological relationships of research subjects, and sample provenance of archival tumor/normal samples. Human sample identification may also be required to identify human mixtures, including cell line contamination, histological sample contamination, mixtures of maternal and fetal samples, or tracking of xenografts.
The GenePrint® 24 System is a multiplex PCR-based genotyping method for research use only. The GenePrint® 24 System includes 22 polymorphic STR markers and two sex markers for sensitive human identification and mixture detection. After amplification, samples can be analyzed using the Spectrum Compact CE System for capillary electrophoresis. With this method, half of the alleles unique to a minor contributor were detected from a mixture containing only 3% minor contributor DNA. The degree of contamination or chimerism can also be estimated from this data based on the peak heights of alleles using ChimerMarker™ Software. This study demonstrates these sensitive applications of human mixture analysis on the Spectrum Compact CE System.
