Cell-Based Assays –
From Basic Principles to Distinct Applications
- select the appropriate cell-based assay
- set-up a cell-based assay and avoid common pitfalls
- improve data quality and cost-efficiency
Summary
This webinar will provide an overview of factors key to the successful implementation of cell-based assays in your lab. Guidance on choosing the optimal plate type and detection mode, cell handling, assay design, and timing will be given with helpful tips and tricks to avoid common pitfalls. The various assay formats available - e.g. single vs. multiplex and endpoint vs. live-cell kinetic analysis - their benefits and limitations will be discussed concerning assays to determine cell viability, cytotoxicity, and apoptosis.
You will learn more about:
- Cell-based assays
- Plate type and detection mode
- Assay design
- Assay timing
- Cell viability
- Cytotoxicity
- Apoptosis
- Endpoint and live-cell kinetic analysis
Speaker
Erik Bonke, PhD
Field Application Specialist
Promega GmbH
Erik Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.