Promega Corporation

PDE-Glo™ Phosphodiesterase Assay

The PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase activity from purified sources. Cyclic nucleotide phosphodiesterases (PDEs) are involved in a myriad of cellular processes due to their ability to hydrolyze, and thus control, the levels of the second-messenger signaling molecules cAMP and cGMP.

The availability of selective inhibitors for PDEs has facilitated their use as tools to study cyclic nucleotide signaling and paved the way to investigate the role of PDEs in cellular and tissue pathologies. The PDE-Glo™ Phospho...

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PDE-Glo™ Phosphodiesterase Assay

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PDE-Glo™ Phosphodiesterase Assay

 Components

  • PDE-Glo™ Reaction Buffer 5X

    V133A1 x 1.2ml
  • PDE-Glo™ Detection Buffer 5X

    V134A1 x 600μl
  • Protein Kinase A

    V135A1 x 20μl
  • PDE-Glo™ Termination Buffer 5X

    V136A1 x 600μl
  • cGMP, 1mM

    V641A1 x 500μl
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
1,000 assays
- V1361 € 426,00 Add to cart

PDE-Glo™ Phosphodiesterase Assay

Close Window

PDE-Glo™ Phosphodiesterase Assay

 Components

  • PDE-Glo™ Reaction Buffer 5X

    V133B1 x 12ml
  • PDE-Glo™ Detection Buffer 5X

    V134B1 x 6ml
  • Protein Kinase A

    V135B1 x 200μl
  • PDE-Glo™ Termination Buffer 5X

    V136B1 x 5ml
  • cGMP, 1mM

    V641A2 x 500μl
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
10,000 assays
- V1362 € 3.827,00 Add to cart


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Storage Conditions

Store the system at –20°C. See the product label for the expiration date.

For product intended use please see Patents & Disclaimers tab.

Representation of the PDE-Glo Phosphodiesterase Assay protocol.Figure 1. Representation of the PDE-Glo™ Phosphodiesterase Assay protocol.

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Use Restrictions

V1361, V1362 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V1361, V1362 Patent Pending.

V1361, V1362 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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