HaloTag® Technology for Protein Interaction Studies

Identify Rare Protein:Protein Interactions

Protein interactions are critical to the majority of cellular processes, including: replication, transcription, translation and signal transduction. The characterization of these interactions aids researchers in determining the function of proteins and the genes that encode them.

• Obtain highly pure protein with minimal effort
• Protein recovery superior to FLAG® and His tags
• Convenient scalability from μg to mg levels
• Quantitate expression levels using fluorescence

Schematic of the HaloTag® Mammalian Pull-Down System protocol

Example of a pull-down experiment detected by silver staining after SDS-PAGE separation.

Superior recovery and purity

Better recovery of highly purified protein compared to FLAG® and His₆ tags

Covalent binding of HaloTag®-fusion proteins affords efficient protein recovery, and low non-specific binding.

Compatible with many downstream applications. Samples can be analyzed by SDS electrophoresis followed by silver stain detection, Western blotting or mass spectrometry (MS).

"Examining the Complexity of Human RNA Polymerase Complexes..."

J Proteome Res., 2012 Jan. 3

Click to read article »