Lumit® IL-1β Human/Mouse Immunoassay
No-Wash Immunoassay for IL-1β Detection in Cells
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a standard plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6010
Catalog Number: W6011
Catalog Number: W6012
Catalog Number: W7010
Catalog Number: W7011
Catalog Number: W7012
Catalog Number: W116A-C
Catalog Number: W119A-C
Quantify Inflammasome Activation Without Tedious Wash Steps
The Lumit® IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.
How the Lumit® IL-1β Immunoassay Works
Simple One-Plate Protocol, No Wash Steps!
Large Dynamic Range of Lumit® Detection
The picomolar to nanomolar linear range of Lumit® detection covers expected concentrations for most cell models.
|
Assay |
IL-1β (Human) Immunoassay |
IL-1β (Mouse) Immunoassay |
|
Limit of Detection (LOD) |
10pg/ml (3 SD above background) |
8pg/ml (3 SD above background) |
|
Dynamic Range |
22pg/ml – 40ng/ml |
11pg/ml – 20ng/ml |
|
Minimal Detectable Dose (MDD) |
7pg/ml (2 SD above background) |
5pg/ml (2 SD above background) |
|
Assay Time |
70 minutes |
|
|
Sample Type |
Cell culture supernatants |
|
SD=Standard Deviation
High-Throughput NLRP3 Inflammasome Inhibitor Screening
The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit® IL-1β (Human) Immunoassay was performed after 5 hours.
Featured Publication
This peer-reviewed study "Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays" examines the direct engagement and functionality of NLRP3 pathway activity in cells using several mechanistic assays, including the Lumit® IL-1β Immunoassay and Caspase-Glo 1 Inflammasome Assay.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
Can’t find your target in the list?
Please enquire about early access to new assays in development.
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117A | 1 × 15μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118A | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117B | 1 × 150μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118B | 1 × 150μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117A | 5 × 15μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118A | 5 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120A | 1 × 15μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121A | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120B | 1 × 150μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121B | 1 × 150μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120A | 5 × 15μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121A | 5 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
Resources
Articles
- Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Application Note: Culturing THP-1 Cells for Inflammasome Activation Assays
- High-Throughput Cytokine Detection with Lumit® Technology
- A Comprehensive Toolkit for NLRP3 Inflammasome Drug Discovery
- Targeted zinc-finger repressors to the oncogenic HBZ gene inhibit adult T-cell leukemia (ATL) proliferation
- Structural basis for thioredoxin-mediated suppression of NLRP1 inflammasome
- ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis
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Frequently Used With
Caspase-Glo® 1 Inflammasome Assay
A homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.
G9951, G9952, G9953
Thaw-and-Use Primary Effector Cells
Consistent and robust method for target cell killing.
CS3055C02, CS3055C03, CS3055B03, CS3055B06, CS3055A15, CS3055A18
Lumit® Immunoassay Labeling Kit and Detection Reagents
Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
