GenePrint® 10 System
STR Analysis for Human Cell Line Authentication
- Tolerates higher DNA amounts. Better balance for aneuploid samples
- Reduced PCR Time. Amplification in less than 1.5 hours
- Lower number of loci simplifies interpretation
Catalog Number:
Size
Catalog Number: B9510
10-Locus STR System for Cell Line Authentication
Cell line misidentification or contamination can derail your research. Fortunately, there is an easy way to confirm the identity of human cell lines in your laboratory: short tandem repeat (STR) analysis.
The GenePrint® 10 System is a 10-locus multiplex system designed to generate a multi-locus DNA profile by amplifying human genomic DNA extracted from cell pellets, cell culture enrichment media and by direct amplification from storage cards.
Economical, Efficient Cell Line Authentication
The GenePrint® 10 System includes all reagents required for successful identification and authentication of human cell lines, as well as detection of cell line contaminants; the small kit size and lower reaction volume makes GenePrint® 10 an economical choice for confirmation of cell line identity.
The GenePrint® 10 System:
- Improves lab efficiencies with rapid-cycling and direct-amplification from storage card samples.
- Simplifies data analysis and comparison using loci in concordance with publicly available databases.
- Generates optimal heterozygote balance using up to 10ng of DNA template.
- Delivers greater consistency in detecting low-level mixtures, contaminants and genetic aberrations.
Configuration of the GenePrint® 10 System
Ready to Use in Your Workflow
Improved Balance with Large Template Input
Amplification of 10ng of human DNA using the GenePrint® 10 System and analyzed with a Spectrum Compact CE System using a 3.0kV, 4-second injection.
Compatible with Direct Amplification
Improved Balance with Large Template Input
Detection Instrument Compatibility and Data Analysis
The GenePrint® 10 System is compatible with the Spectrum Compact CE System and the Applied Biosystems® SeqStudio, 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation.
Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® and GeneMarker® software and are available for download.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
GenePrint® 10 5X Primer Pair Mix |
B934A | 1 × 250μl | ||
GenePrint® 10 5X Master Mix |
B935A | 1 × 250μl | ||
GenePrint® 10 Allelic Ladder Mix |
B937A | 1 × 50μl | ||
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
Internal Lane Standard 600 |
DG107A | 1 × 150μl | View Product | |
|
Water, Amplification Grade |
DW099A | 1 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
Related Products
Frequently Used With
Spectrum Compact CE System
An affordable benchtop instrument for Sanger sequencing and fragment analysis.
CE1304, CE1307, CE1305
GenePrint® 5C Matrix Standard
Matrix fragments for spectral calibration when using the GenePrint® 24 System.
B1930
PowerPlex® Matrix Standards
DNA fragments labeled with fluorescent dyes for use with the PowerPlex® Systems.
DG4800, DG4850, DG4900, DG5010
