NotI
- Capable of digesting DNA in 15 minutes or less
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6431
Catalog Number: R6435
Cut Site:
GCâ–¼GGCC Â GC
CGÂ Â CCGGâ–²CG
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.
Incubation Conditions: Buffer D. 37°C.
Source: Nocardia otitidis-caviarum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-COREâ„¢ |
|---|---|---|---|---|
| <10% | 10–25% | 25–50% | 100% | 25–50% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 0 | 7 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
NotI |
R643A | 1 × 200u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
NotI |
R643E | 1 × 1,000u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
Related Products
Frequently Used With
LigaFastâ„¢ Rapid DNA Ligation System
Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.
M8221, M8225
Blue/Orange Loading Dye, 6X
Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.
G1881
Agarose, LE, Analytical Grade
Reagent for electrophoretic separation of nucleic acids.
V3121, V3125
