Lumit® Immunoassay Cellular Systems
No-Wash, One-Plate Immunoassay for Cellular Analytes
- New Lumit® Lysis Buffer II included! Offers enhanced performance across a wide range of cellular targets
- New Application Notes available for more validated targets
- Set 1: Lumit® Anti-Mouse Ab-LgBiT/Lumit® Anti-Rabbit Ab-SmBiT
- Set 2: Lumit® Anti-Mouse Ab-SmBiT/Lumit® Anti-Rabbit Ab-LgBiT
- Starter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user
Catalog Number:
Choose a System
Size
Catalog Number: W1201
Catalog Number: W1202
Catalog Number: W1203
Catalog Number: W1331
Catalog Number: W1332
Catalog Number: W1333
Catalog Number: W1220
Fast Quantification of Cellular Analytes
The Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect a wide range of proteins, phosphoproteins or small molecules of interest, provided the appropriate primary antibodies (supplied by the user) are available. We recently developed a new lysis buffer (Lumit® Lysis Buffer II), which broadens the range of cellular targets (see table below) that can be interrogated using the Lumit® Immunoassay Cellular System.
Also available are Complete Assays, which include sets of antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK as well as several others. These are available as early access materials.
To save you time, we have evaluated a number of common targets using commercially available primary antibodies. We have expanded the number of Application Notes to include targets that are now accessible due to the new lysis buffer. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or up to 4,000 assays in 384-well plates.
- 10,000 assay kits: applicable for 10,000 assays in 96-well plates or 40,000 assays in 384-well plates.
Cellular Pathway Analysis Series
*New Lysis Protocol using Lumit® Lysis Buffer II.
| Human Cellular Target | Signaling Pathway | Lumit® Immunoassay Cellular System (Cat.#) |
Application Note / Complete Kit |
|---|---|---|---|
| Phospho-4E-BP1 (Ser 65) | mTor | Set 2 (W1331) | App Note |
 AKT |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note |
| Phospho-AKT (Ser 473) | PI3K/mTOR/AKT | Set 1 (W1201) | App Note*/ Complete Kit |
| BCL6 | B-cell signaling | Set 1 (W1201) | App Note |
| β-catenin (Human) | WNT | Set 2 (W1331) | App Note |
| Phospho-β-catenin (Thr 41/Ser 45) | WNT | Set 2 (W1331) | App Note |
| Burton's Tyrosine Kinase (BTK) |
B-cell Receptor (BCR) | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-BTK (Tyr 223) | B-cell Receptor (BCR) | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-CHK1 (Ser 317) |
DDR Pathway | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-c-Jun (Ser 63) | JNK | Set 1 (W1201) | App Note |
| Phospho-c-MET (Tyr 1234/1235) |
c-MET | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-c-MET (Tyr 1349) |
c-MET | Set 1 (W1201) | App Note |
| CREB | PKA/CREB | Set 1 (W1201) | App Note |
| Phospho-CREB (Ser 133) | PKA/CREB | Set 1 (W1201) | App Note |
| EGFR |
EGFR | Set 1 (W1201) | Complete Kit |
| Phospho-EGFR (Tyr 1068) |
EGFR | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-EGFR (Tyr 1173) |
EGFR | Set 1 (W1201) | App Note |
| ER (Estrogen Receptor) | Estrogen | Set 1 (W1201) | App Note |
| Phospho-ERK1 (Thr 202) | ERK | Set 1 (W1201) | App Note*/ Complete Kit |
| Phospho-GSK1-3β (Ser 9) |
GSK-3β | Set 1 (W1201) | App Note |
| Phospho-H2AX |
DDR Pathway | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-HER2 (Tyr 1196) |
HER2 | Set 1 (W1201) | App Note |
| Phospho-HER2 (Tyr 1221/1222) |
HER2 | Set 1 (W1201) | App Note |
| IκBα | NF-κB | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-IκBα (Ser 32) | NF-κB | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-JNK (Thr 183/Tyr 185) | JNK | Set 1 (W1201) | App Note*/ Complete Kit |
| p65 | NF-κB | Set 2 (W1331) | App Note* |
| Phospho-p65 (Ser 536) | NF-κB | Set 2 (W1331) | App Note*/ Complete Kit |
| Rb (Retinoblastoma Tumor Suppressor) | Cell Cycle | Set 2 (W1331) | App Note |
| Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 780) | Cell Cycle | Set 2 (W1331) | App Note |
| Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 807/811) | Cell Cycle | Set 2 (W1331) | App Note/ Complete Kit |
| Ribosomal Protein S6 |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note |
| Phospho-Ribosomal Protein S6 (Ser 240/244) |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note/ Complete Kit |
| Smad1 | BMP | Set 1 (W1201) | App Note |
| Phospho-Smad1 (Ser 463/465) | BMP | Set 1 (W1201) | App Note |
| Smad2 | TGF-β | Set 2 (W1331) | App Note |
| Phospho-Smad2 (Ser 465/467) | TGF-β | Set 2 (W1331) | App Note |
| STAT1 | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT1 (Tyr 701) | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT1 (Ser 727) | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT2 (Tyr 690) | JAK/STAT | Set 1 (W1201) | App Note |
| STAT 3 |
JAK/STAT | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-STAT 3 (Tyr 705) | JAK/STAT | Set 1 (W1201) | App Note/ Complete Kit |
Targeted Protein Degradation Series
Learn more about our solutions for targeted protein degradation.
| Human Cellular Target | Degrader(s) | Lumit® Immunoassay Cellular System (Cat.#) |
Application Note / Complete Kit |
|---|---|---|---|
| AKT |
PROTAC MS-21 | Set 1 (W1201) | App Note |
| B-cell lymphoma 6 protein (BCL-6) | BI-3802 | Set 1 (W1201) | App Note |
| BRD4 |
PROTACs ARV-771, dBET6 | Set 1 (W1201) | App Note/ Complete Kit |
| Burton's Tyrosine Kinase (BTK) |
PROTAC MT-802 | Set 1 (W1201) | App Note/ Complete Kit |
| ER (Estrogen Receptor) | Fulvestrant | Set 1 (W1201) | App Note |
| SMARCA2 (BRM) |
PROTAC ACBI-1 | See Application Note for details | App Note/ Complete Kit |
| SMARCA4 (BRG1) |
PROTAC ACBI-1 | Set 2 (W1331) | App Note/ Complete Kit |
| STAT3 |
PROTAC SD-36 | Set 1 (W1201) | App Note/ Complete Kit |
How the Lumit® Immunoassay Cellular System Works
Simple One-Plate Protocol, No Wash Steps!
The Lumit® Immunoassay Cellular System does not require media removal or lysate transfer. The protocol simply involves addition of the labeled antibodies to the sample, addition of the detection reagent and reading of the luminescent signal—all in a single plate!
Lumit® Immunoassay vs. Western Blot
Lumit® Immunoassays are easier and much faster than traditional Western blots while generating comparable data.
Quantitative Detection of Total and Phosphorylated Proteins
Using Lumit® Immunoassay Cellular Systems for Screening Novel Compounds in a Drug Discovery Process
Here, Dr. Tracey Martin from Cardiff University School of Medicine describes use of Lumit® Immunoassays in her work studying the motility protein N-WASP. The project involves screening 150 novel compounds to assess their effects on N-WASP expression and phosphorylation status. Multiple concentrations of each compound are tested in different cell types, generating a large number of samples. Originally the team were using Western blotting to look at N-WASP levels, however this was found to be too expensive and both labor and time-intensive.
See More Data: This peer-reviewed paper published in Nature Communications Biology demonstrates how Lumit® Immunoassays were used to monitor multiple signaling pathway activation through specific nodes of phosphorylation (pIκBα, pAKT and pSTAT3).
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 1 × 50μl |
Lumit® Detection Substrate C |
VB103A | 1 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 1 × 500μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 1 × 500μl |
Lumit® Detection Substrate C |
VB103B | 2 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 1 × 15ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102B | 1 × 300μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103B | 1 × 300μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 2 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 1 × 5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 10 × 500μl |
Lumit® Detection Substrate C |
VB103B | 20 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 10 × 15ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102B | 10 × 300μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103B | 10 × 300μl |
Antibody Dilution Buffer |
W108A | 10 × 1ml |
|
GF-AFC Substrate |
G608A | 20 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 10 × 5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 1 × 50μl |
Lumit® Detection Substrate C |
VB103A | 1 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 1 × 30μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 1 × 500μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 1 × 500μl |
Lumit® Detection Substrate C |
VB103B | 2 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 1 × 15ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104B | 1 × 300μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105B | 1 × 300μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 2 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 1 × 5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 10 × 500μl |
Lumit® Detection Substrate C |
VB103B | 20 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 10 × 15ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104B | 10 × 300μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105B | 10 × 300μl |
Antibody Dilution Buffer |
W108A | 10 × 1ml |
|
GF-AFC Substrate |
G608A | 20 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 10 × 5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 2 × 50μl |
Lumit® Detection Substrate C |
VB103A | 2 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 2 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 1 × 30μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 2 × 500μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
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