The GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line is a clonal derivative of Human Embryonic Kidney 293 (HEK293) cells. These cells contain a luciferase gene (luc2P) under the control of a minimal TATA promoter with multiple Nuclear Factor-κB response elements (NF-κB-REs). NF-κB-REs are the DNA-binding sequences for the NF-κB transcription factor, which is responsible for regulating inflammation, immune response, cell growth and apoptosis. The GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line is designed for rapid and convenient analysis of any cellular response that results in modulation of NF-κB activities.
The GloResponse™ Cell Lines incorporate the improvements developed for the pGL4 family of reporter vectors for enhanced performance. The destabilized luc2P luciferase reporter is used for improved responsiveness to transcriptional dynamics. The luc2P gene is codon optimized for enhanced expression in mammalian cells, and the pGL4 plasmid backbone was engineered to reduce background reporter expression. The result is a cell line with very high induction levels when the pathway of interest is activated.
GloResponse™ NF-κB-RE-luc2P HEK293 cells response to TNF-α titration. A total of 10,000 GloResponse™ NF-κB-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and twofold serial dilutions of TNF-α were added to induce reporter gene expression. After 5 hours of induction in a tissue culture incubator, luciferase activity was quantified using the ONE-Glo™ Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
NF-κB-luc2P HEK293 Cell Line |
E852A | 2 × 1 vial |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.
HEK293 cells were obtained under license from AdVec Inc.
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Frequently Used With
Steady-Glo® Luciferase Assay System
A sensitive reporter assay with long-lived luminescence for batch processing in multiwell plates.
E2510, E2520, E2550
ONE-Glo™ Luciferase Assay System
Robust firefly luciferase assay with improved performance for high- and ultrahigh-throughput applications.
E6110, E6120, E6130
Luciferase Assay System
Sensitive detection of firefly luciferase activity. Requires a luminometer with one injector.
E1500, E4030, E1501, E4530, E4550
GloMax® Navigator System
Dedicated microplate luminometer.
GM2000, GM2010
