Detect Luciferase Reporter Gene Expression in Reporter Bioassays
The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay. Bio-Glo™ Assay reagent contains a new luciferase substrate, resulting in a reagent that is more stable and more tolerant to sample components than standard luciferase assay reagents. Bio-Glo™ Assay reagent is functionally tested for performance in the ADCC Reporter Bioassay and is intended for use with this or other bioassays.
Bio-Glo™ Luciferase Assay System Uses a Novel Firefly Luciferase Substrate, 5´-fluoroluciferin.
Advantages
Simplified Assay Optimization: Robust performance, improved storage and convenient size.
Improved Assay Precision: The Bio-Glo™ Reagent is less sensitive to mixing and dispensing conditions, enhancing reproducibility. Ideal for bioassay applications.
Brighter, Longer-Lasting Signal: Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations.
Reduced Unwanted Effects from Sample Components: The Bio-Glo™ Assay is less sensitive to culture media, phenol red and luciferase inhibitors than other luciferase assays.
Applications
For use in the ADCC Reporter Bioassay.
For use in reporter-based bioassays.
Available for Bulk and Custom Purchase
For information on bulk purchases and custom configurations, please contact COD@promega.com
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Bio-Glo™ Luciferase Assay Buffer |
G719B | 1 × 100ml |
| Bio-Glo™ Luciferase Assay Substrate | G720B | 1 × 1 vial |
SDS
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Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
Bio-Glo™ Luciferase Assay Buffer |
G719A | 1 × 10ml |
Bio-Glo™ Luciferase Assay Substrate |
G720A | 1 × 1 vial |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
Related Products
Frequently Used With
ADCC Reporter Bioassay, V Variant
Measure potency and stability of antibodies mediating ADCC through the high-affinity human FcγRIIIa-V receptor.
G7015, G7010, G7016, G7018, G7102, GA1130
ADCC Reporter Bioassay, F Variant
Characterize Fc effector activity of antibodies mediating ADCC through the low-affinity human FcγRIIIa-F receptor.
G9790, G9798, G9302
PD-1/PD-L1 Blockade Bioassay
Measure the ability of biologics to block immune checkpoint signals
J1250, J1255, J1252, J1191, J1195, J1201
FcγRIIa-H ADCP Bioassay
Characterize the Fc effector activity of antibodies that bind and activate the human FcγRIIa-H receptor.
G9901, G9991, G9995, G9871