ISOQUANT® Isoaspartate Detection Kit Technical Bulletin
Instructions for Use of Product(s)
MA1010
Literature # TBI001
The ISOQUANT® Isoaspartate Detection Kit is intended for quantitative detection of isoaspartic acid residues in proteins and peptides, which can result from the gradual, nonenzymatic deamidation of asparagine or rearrangement of aspartic acid residues during storage or handling.
The ISOQUANT® Kit provides reagents for deamidation detection using an HPLC method. The amount of isoaspartic acid to target protein is determined by comparison with reactions performed using a reference standard peptide.
Summary of Changes
The following changes were made to the 12/24 revision of this document:
1. In Section 3.E, Step 3, storage conditions for stopped reactions were updated.
2. Technical bulletin cover, template and font were updated.
3. Minor formatting changes were made, such as changing bullet points under “Notes:” to a., b., etc.
Revised 12/24.