Epigenetik
Promega Kits und Reagenzien für die Epigenetik-Forschung. DNA-Methylierungsanalyse, PCR und qPCR, Luciferase-Reporterassays, HDAC Assays und HaloTag-Proteinaufreinigungssysteme.
Epigenetik Produktgruppen
Epigenetische Assays
Lumineszente Assays zum Nachweis von HDAC, SIRT, Methyltransferasen und weiteren Proteaseaktivitäten, sowie für epigenetische Studien relevante Proteininteraktionsassays.
Methylierungsassays
Methylase-Aktivitätsassays, DNA-Aufreinigungskits, PCR-Reagenzien, methylierungssensitive Restriktionsenzyme und weitere Werkzeuge für die Transfektion und Reporterassays.
Proteinaufreinigung und Analyse von Protein Interaktionen
Proteinaufreinigungs- und Interaktionskits zur Analyse von Histonmodifikationen und schnellen und effizienten Bestimmung von Proteinbindungspartnern.
Epigenetik Produkte für Ihr Labor
NanoBRET® Target Engagement HDAC Assays
Directly measure HDAC-test compound affinity and residence time in live cells.
N2080, N2081, N2170, N2090
MTase-Glo™ Methyltransferase Assay
Measure methyltransferase activity using a universal bioluminescent assay.
V7601, V7602
HaloTag® Protein Purification System
Enhanced expression and solubility of recombinant proteins with HaloTag® Ligands.
G6280, G6270
Epigenetik Basics
Epigenetics is the study of heritable changes in gene expression that arise from changes in chromosomes without alteration of DNA sequence. The coordination between DNA methylation and silencing histone marks leads to compaction of chromatin and gene repression.
The methylation status of a DNA sequence can be determined using a variety of techniques, such as restriction enzymes that are sensitive to methylation. Bisulfite sequencing is another way to assess DNA methylation. After bisulfite conversion and DNA cleanup, the DNA is amplified by whole genome PCR, and the amplified products are analyzed using one of several methods.The firefly luciferase reporter protein (Fluc) can also be used to assess DNA methylation.
To screen for potential histone deacetylase (HDAC) inhibitors, Promega offers the HDAC-Glo™ I/II and SIRT-Glo™ Assays to measure relative activities of HDAC class I and II enzymes and sirtuins, respectively. Promega also offers a number of kinase enzyme systems to monitor the activity or identify inhibitors of different kinases involved in histone phosphorylation.
